以不同产地人工林中采取的降香黄檀( Dalbergia odorifera T. Chen)木材为研究对象,提取并扩增不同温度处理后的降香黄檀心、边材DNA和ITS片段,分析不同温度处理对降香黄檀木材DNA提取和PCR扩增的影响；对不同产地的降香黄檀木材及其近缘种多裂黄檀( Dalbergia rimosa Roxb)木材的rDNA-ITS序列进行测定和差异分析。结果表明,25℃和65℃热处理后的木材DNA呈不同程度的弥散分布,105℃热处理后的木材DNA降解成250bp以下的小片段；不同温度处理后的降香黄檀木材,仅有25℃处理后的木材ITS片段能够被成功地扩增。降香黄檀和多裂黄檀ITS序列共存在6个变异位点且ITS2区变异大于ITS1区,聚类分析结果可以将降香黄檀及其近缘种多裂黄檀木材区分开来,为利用ITS条形码序列鉴定降香黄檀木材及其常见混伪品提供理论依据。

The wood of Dalbergia odorifera T. Chen originated from different sites of plantations were sampled as materials in this paper. DNA from the sapwood and heartwood of D. odorifera treated with various temperature was extracted and amplified for the internal transcribed space ( ITS) sequences. The effects of various temperature treatments on DNA extraction and amplification for target sequences were analyzed. The rDNA-ITS sequences of D. odorifera from different habitats and D. rimosa Roxb, a closely related species, were sequenced and their divergences were analyzed. The results showed that there were varying extents of diffusion with the DNA extracts from the heat treatment of 25℃ and 65℃, and the DNA from the heat treatment of 105℃ was degraded and smaller than 250bp. The ITS sequences can be amplified successfully from wood after 25℃ heat treatments. The distribution of vriations in ITS2 regions of D. odorifera and D. rimosa was larger than ITS1 regions and there were 6

对降香黄檀及市场上5种假冒降香黄檀木材进行了取样及实验室解剖，查阅了相关书籍资料，对降香黄檀及假冒木材的树木性状、木材构造特征、材性和用途进行了描述，从木材宏观和微观构造的角度浅析了降香黄檀与假冒木材之间的天壤之别，其结果表明：降香黄檀木材具有独特的辛辣香气，而5种假冒树种均无特殊气味；肉眼下，5种假冒木材与降香黄檀相似，其纹理和色泽均多变，鬼脸或不规则花纹均可见；肉眼下，降香黄檀弦切面具明显波痕，而5种假冒木材均无波痕；微观下，降香黄檀导管、木射线、轴向薄壁组织均叠生，而5种假冒木材均不叠生。以期望为大众提供识别依据。

T his article expects to provide an identification basis for the public to analyze the profound differ-ence between Dalbergiaodorifera wood and the fake one through some efforts including sampling and dissec-tion research of Dalbergiaodorifera wood and five kinds of fake ones in the market ,checking the relevant books and resources ,describing Dalbergiaodorifera wood and fake one''s characters ,structure features ,proper-ties and usages ,and analyzing from the perspective of macro -structure and micro -structure .T he results can be concluded as follows :(1)Dalbergiaodorifera has a unique spicy aroma ,but five kinds of fake wood have no special smell ;(2)Observed by the naked eye ,five kinds of fake wood are similar to Dalbergiaodorif-era wood ,but their texture and color are changeable and grimace or irregular pattern are visible ;.(3)Under the naked eye ,section of Dalbergiaodorifera presents obvious wavemark ,but five kinds of fake wood have none .(4)Under the microscope ,

用6-BA、IBA、GA3等3种植物生长调节剂对降香黄檀种子进行萌发处理,测定降香黄檀种子中可溶性糖、淀粉、蛋白质和脂肪含量的变化。结果表明,降香黄檀种子在不同的萌发处理过程中,可溶性糖含量均呈下降趋势,萌发结束时淀粉含量由第3 d的0.6%下降至0.2%以下,其中6-BA处理后种子萌发前期(3~6 d)淀粉含量变化不大,后期则有一个显著的下降趋势;3种植物生长调节剂对降香黄檀种子蛋白质、脂肪含量变化影响不明显。

Seeds of Dalbergia odorifera were treated by three plant growth regulators of 6-BA, IBA, GA 3 and distilled water. The content of soluble sugar, starch, protein and fat of seeds were determined during germination. The results showed as follows:seeds of D. odorifera showed a downward trend in the soluble sugar content in all treatments. The starch content dropped from 0.6%which was the one of the third day to 0.2% below when the seeds ended germination. In the early period of germination (3~6 d), starch content of seeds changed little with the treatment of 6-BA, but there was a significant downward trend in the later period of germination. Changes of protein and fat content were relatively stable, and inapparent after treatment of three plant growth regulators.

为了加快降香黄檀心材形成的速度，采用乙烯利诱导降香黄檀形成心材，水蒸气蒸馏法提取心材的挥发油，利用GC-MS对提取的挥发油进行化学成分分析。结果表明：从挥发油中，鉴别出8种化学成分，含量最高的是橙花叔醇（39.06%）。说明乙烯利诱导能够促进降香黄檀心材形成，是一种值得深入研究和推广使用的方法。

To analyze the chemical compositions of essential oils extacted from aromatic heartwood in Dalbergia odorifera T. Chen by ethephon stimulation. The essential oils of Dalbergia odorifera were extracted by steam distillation extraction. The chemical components were separated and analyzed by gas chromatography-mass spectrometry. The results indicated 8 compounds were identified in essential oils. The major components from essential oils were nerolidol ( 39.06%) . Ethephon stimulation can accelerate the heartwood formation of Dalbergia odorifera T. Chen , which is a method of worth exploring and promoting.

根据降香黄檀的生物学和生态学特性以及引种地的环境条件，通过引种栽培试验，降香黄檀在藤县大芒界林场四旁及周边丘陵山地生长表现正常，造林成活率达98％以上，保存率达97％以上，平均树高达2．0m以上，平均地径达1．1cm以上，未发现冻害，能安全过冬，对气候适应性较强，为在藤县乃至梧州推广种植提供参考。

According to the biological and ecological characteristics of Dalbergia odori f era and the environmental conditions of introduction ,by the test of introduction and cultivation , Dalbergia odori f era was found growing normally in the “four sides”of Tengxian Damangjie forestry centre or Hills and Mountains all around .The survival rate of afforestation is 98% ,the preserve rate is 97% ,the average height is 2 .0m and the ground diameter is 1 .1cm .The freezing injury is not found ,and Dalbergia odori f era could live over winter .Dalbergia odori f era is adaptability to climate .Test results provides reference for planting in Tengxian and Wuzhou .